VALIDATION OF DOUBLE PLATELET COLLECTION BY APHERESIS WITH ADDITIVE PLATELET SOLUTION IN CENTRAL-WESTERN BRAZIL

Platelet additive solution (PAS) is added to platelet concentrate in transfusion services for patient management and inventory optimization. The reduction in plasma volume and the low isoagglutinin titer reduce immediate transfusion reactions. In the Center-West of Brazil, for the first time this methodology will be applied to all patients in the public and private network. The study validated the collection of platelet concentrates by apheresis with PAS, using equipment whose cell separation is by elutriation, returning residual leukocytes to the donor, ensuring safety for frequent donors. The research involved 37 platelet donations from occasional voluntary donors, with platelet counts ≥ 200,000 cells/uL and body surface ≥ 1.7 m2. The AMICORE 2.1 equipment was analyzed for its performance for double collection with the addition of Intersol (PAS). All donors signed a Consent Form recording the risks and authorizing the disclosure of data. The collection protocol adjusted the double yield of platelets considering the maximum time spent in the equipment of 120 minutes. The citrate infusion rate was adjusted according to donor sensitivity. An immediate pre- and post-donation sample was collected from the donor to count platelets and leukocytes in a differential counter. Platelets counts were performed in the Neubauer chamber and leukocyte counts in the Nageotte chamber. In all platelet concentrates, blood culture was performed for aerobic, anaerobic and fungal microorganisms and the frequency of immediate transfusion reactions was verified. The perception of the equipment operator was verified through 2 questions with answers on a scale of 1 to 10. In relation to the donor, we evaluated the reduction in platelet and leukocyte counts after donation and incidents during the procedure. The concentrated platelets were stored in two bags, designed under continuous temperature of 20 to 24ºC and the samples were separated from the bag, without opening the system, for quality control immediately before transfusion to be evaluated up to the 5th day of storage. Of the 37 donations selected, 1 (2.7%) was excluded because the donor had to withdraw unexpectedly due to personal problems. The analyzed data (n = 36) are shown in Table 1: Results of performance qualification in collecting plates from the AMICORE 2.1 equipment - kit 6R8884 with the addition of Intersol* (PAS). The equipment was qualified for double platelet collection by apheresis. The low reduction of leukoc