Changes in sputum microbiota during treatment for nontuberculous mycobacterial pulmonary disease

Limited data exist on longitudinal changes in the sputum bacterial microbiome during treatment in nontuberculous mycobacterial pulmonary disease (NTM-PD) patients. We prospectively collected serial sputum samples from 14 NTM-PD patients during treatment, at the start (n = 14) and at 1 (n = 10), 3 (n...

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Veröffentlicht in:Scientific reports 2023-11, Vol.13 (1), p.19764-19764, Article 19764
Hauptverfasser: Kim, Bo-Guen, Yu, Jin Young, Kim, Su-Young, Kim, Dae Hun, Jhun, Byung Woo
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Sprache:eng
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Zusammenfassung:Limited data exist on longitudinal changes in the sputum bacterial microbiome during treatment in nontuberculous mycobacterial pulmonary disease (NTM-PD) patients. We prospectively collected serial sputum samples from 14 NTM-PD patients during treatment, at the start (n = 14) and at 1 (n = 10), 3 (n = 10), 6 (n = 12), and 12 (n = 7) months. The bacterial microbiome changes were analyzed using 16S rRNA sequences (V3–V4 regions). Subgroup analysis included culture conversion (n = 9) and treatment refractory (n = 5) groups. In all patients, sputum alpha-diversity (ACE, Chao1, and Jackknife) significantly decreased during antibiotic treatment at 1, 3, 6, and 12 months compared to treatment initiation levels. Within the culture conversion group, genus/species-level beta-diversity showed differences at 1, 3, 6, and 12 months compared to treatment initiation (all p  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-47230-5