BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION

BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION

The optimization of the conditions of bacterial expression of recombinant tyrosyl-tRNA synthetase was conducted. The influence of the concentration of synthesis inductor of target protein at its final output and the best time of cultivation of bacterial culture before and after inductor adding was i...

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Veröffentlicht in:Mìkrobìologìâ ì bìotehnologìâ 2009-12 (4(8)), p.6-12
Hauptverfasser: Кондратюк, Ю. Ю., Бабарик, М. А., Корнелюк, О. I.
Format: Artikel
Sprache:eng
Schlagworte:
бактеріальна система експресії
оптимізація експресії
тирозил-трнк синтетаза
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Zusammenfassung:The optimization of the conditions of bacterial expression of recombinant tyrosyl-tRNA synthetase was conducted. The influence of the concentration of synthesis inductor of target protein at its final output and the best time of cultivation of bacterial culture before and after inductor adding was investigated. The optimal conditions for culture E. coli BL21 (DE3) pLysE cultivation to achieve a high expression level of recombinant protein were proposed.
ISSN:2076-0558
2307-4663
DOI:10.18524/2307-4663.2009.4(8).103197