Extraction, Purification and Therapeutic Use of Bacteriophage Endolysin against Multi-Drug Resistant Staphylococcus aureus: in-vivo and in-vitro study

Objectives: Isolation of endolysin from Staphylococcus aureus bacteriophages, and administering them systemic In vivo lab animal and measure the therapeutic efficacy as well as evaluation of their biosafety. Method: This study was performed from March 2015 – August 2017, 50 bacteriological samples of S. aureus were collected , and examined with their antibiogram, then bacteriophage cocktails were done for 5 resistant strains of them. Endolysins were extracted from their corresponding bacteriophages, they were characterized and the enzymatic and antibacterial activities assays were performed on them in addition to in vivo trial on animals regarding the therapeutic effect of these extracted endolysins on laboratory mice. Results: This study showed that the extracted endolysin from these bacteriophages was effective in treating laboratory mice from bacteremia with S. aureus and saving their lives when injected intraperitoneal. Conclusion: Endolysin can be extracted directly from their bacteriophages and used by injection of mice with bacteremia with the proper dose of the extracted endolysin of the corresponding bacteriophages which was effective in all of them.