Prebiotic potential of a coffee silverskin extract obtained by ultrasound-assisted extraction on Lacticaseibacillus paracasei subsp. paracasei

[Display omitted] •The soluble dietary fiber of coffee silverskin extract is mostly composed of uronic acids.•5-CQA is the main caffeoylquinic acid in coffee silverskin extract.•Coffee silverskin extract enhances Lacticaseibacillus paracasei subs. paracasei growth.•L. paracasei used the coffee silverskin extract, producing acidic metabolites. Prebiotic demand is increasing due to growing concerns about human gut health and well-being. Recently, agri-food by-products have been explored as a potential source of prebiotic ingredients. This study analysed the chemical composition of a freeze-dried coffee silverskin extract (FSE) and evaluated its impact on Lacticaseibacillus paracasei subsp. paracasei BAA-52 ATCC growth. The FSE was obtained by multi-frequency multimode modulation ultrasonic vibration. Soluble dietary fiber accounted for 15.2 % of the extract, being mainly composed of uronic acids (58 mol%) and glucose (13.4 mol%). HPLC-DAD-MS-ESI+ and HPLD-DAD analyses revealed a phenolic-rich composition (mainly caffeoylquinic acids), as well as the presence of caffeine, and 5-hydroxymethylfurfural. The probiotic was incubated in De Man, Rogosa, and Sharpe broth supplemented with different concentrations of the FSE (1–4 %, w/v). The 2 % and 4 % (w/v) concentrations significantly enhanced L. paracasei subs. paracasei growth and decreased the pH of the medium, indicating its potential as a prebiotic ingredient.