Simultaneous Determination of 17 β-Receptor Agonists in Meat by QuEChERS EMR-Lipid with Isotope Dilution-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

Objective: A method for simultaneous determination of 17 β-receptor agonists in meat was established by QuEChERS EMR-Lipid with isotope dilution-ultra performance liquid chromatography-tandem mass spectrometry. Methods: The samples were added to ammonium acetate buffer solution (pH5.2) and enzymatic hydrolyzed by β-glucuronidase and arysulfatase. The hydrolysates were extracted by acetonitrile containing 5% formic acid, and purified by QuEChERS EMR-Lipid. The analyses were separated by CORTECS™ UPLC® C18 chromatographic column with gradient elution using 0.1% formic acid and methanol as the mobile phase. The target compounds were monitored in scheduled MRM mode. Then the internal standard method was used for quantitative analysis. Results: The linear regression correlation coefficients for the 17 β-receptor agonists were all higher than 0.99 in the range from 0 ng/mL to 20 ng/mL. The limits of detection were 0.01~0.09 μg/kg and the limits of quantification were 0.05~0.31 μg/kg. The average recoveries at three