Cytotoxicity of polymers intended for the extrusion-based additive manufacturing of surgical guides

Cytotoxicity of polymers intended for the extrusion-based additive manufacturing of surgical guides

Extrusion-based printing enables simplified and economic manufacturing of surgical guides for oral implant placement. Therefore, the cytotoxicity of a biocopolyester (BE) and a polypropylene (PP), intended for the fused filament fabrication of surgical guides was evaluated. For comparison, a medical...

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Veröffentlicht in:Scientific reports 2022-05, Vol.12 (1), p.7391-7391, Article 7391
Hauptverfasser: Burkhardt, Felix, Spies, Benedikt C., Wesemann, Christian, Schirmeister, Carl G., Licht, Erik H., Beuer, Florian, Steinberg, Thorsten, Pieralli, Stefano
Format: Artikel
Sprache:eng
Schlagworte:
631/80
692/308
Apoptosis
Cell proliferation
Cytotoxicity
Dental implants
Esters
Fabrication
Gene expression
Gene regulation
Gingiva
Humanities and Social Sciences
Humans
Impedance
Inflammation
Keratinocytes
multidisciplinary
Polymerase chain reaction
Polymers
Polypropylene
Polypropylenes - toxicity
Science
Science (multidisciplinary)
Stereolithography
Variance analysis
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Zusammenfassung:Extrusion-based printing enables simplified and economic manufacturing of surgical guides for oral implant placement. Therefore, the cytotoxicity of a biocopolyester (BE) and a polypropylene (PP), intended for the fused filament fabrication of surgical guides was evaluated. For comparison, a medically certified resin based on methacrylic esters (ME) was printed by stereolithography (n = 18 each group). Human gingival keratinocytes (HGK) were exposed to eluates of the tested materials and an impedance measurement and a tetrazolium assay (MTT) were performed. Modulations in gene expression were analyzed by quantitative PCR. One-way ANOVA with post-hoc Tukey tests were applied. None of the materials exceeded the threshold for cytotoxicity (
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-11426-y