Klebsiella pneumoniae Isolates from Meningitis: Epidemiology, Virulence and Antibiotic Resistance

Klebsiella pneumoniae (KP) resistance to broad-spectrum cephalosporin (BSC) in meningitis is important because of limited therapeutic options. To investigate the antibiotic resistance, virulence and epidemiology of KP in meningitis, we conducted a retrospective study for 33 non-metastatic isolates, including primary meningitis ( n = 20) and post-craniotomy meningitis ( n = 13) collected from 1999 to 2013. BSC resistance was found in 9 (27.3%) isolates, all from post-craniotomy meningitis, harboring bla SHV-5 ( n = 6), bla CMY-2 ( n = 2), bla DHA-1 ( n = 2), and bla TEM-1B ( n = 1). Positive virulence factors were hypermucoviscosity ( n = 22), larger bacterial size ( n = 24), virulent capsule serotypes ( n = 24, K2, 11; K1, 5; K57, 3; K5, 2; K20, 2 and K54, 1), rmpA ( n = 23), rmpA 2 ( n = 20), aerobactin gene ( n = 22) and high-grade serum resistance ( n = 23, 69.7%). Higher mouse lethality (LD 50