Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC

Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC

This work demonstrated a method combining reversed-phase high-performance liquid chromatography (RP-HPLC) with chemometrics analysis to identify the authenticity of . The fingerprint chromatograms of the protein were established through an Agilent Zorbax 300SB-C8 column and diode array detection at...

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Veröffentlicht in:Molecules (Basel, Switzerland) Switzerland), 2019-04, Vol.24 (9), p.1687
Hauptverfasser: Gan, Yuanshuai, Xiao, Yao, Wang, Shihan, Guo, Hongye, Liu, Min, Wang, Zhihan, Wang, Yongsheng
Format: Artikel
Sprache:eng
Schlagworte:
Acetonitrile
Chinese medicine
Chromatography
Clustering
Counterfeit
Counterfeiting
fingerprint
Fingerprints
High performance liquid chromatography
Identification
Liquid chromatography
Principal components analysis
protein
Proteins
Ranae Oviductus
RP-HPLC
Software
Toads
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Zusammenfassung:This work demonstrated a method combining reversed-phase high-performance liquid chromatography (RP-HPLC) with chemometrics analysis to identify the authenticity of . The fingerprint chromatograms of the protein were established through an Agilent Zorbax 300SB-C8 column and diode array detection at 215 nm, using 0.085% TFA ( / ) in acetonitrile (A) and 0.1% TFA in ultrapure water (B) as mobile phase. The similarity was in the range of 0.779-0.980. The fingerprint chromatogram of showed a significant difference with counterfeit products. Hierarchical clustering analysis (HCA) and principal component analysis (PCA) successfully identified from the samples. These results indicated that the method established in this work was reliable.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24091687