Overexpression of peanut ( Arachis hypogaea L.) AhGRFi gene enhanced root growth inhibition under exogenous NAA treatment in Arabidopsis thaliana

The 14-3-3 protein is a kind of evolutionary ubiquitous protein family highly conserved in eukaryotes. Initially, 14-3-3 proteins were reported in mammalian nervous tissues, but in the last decade, their role in various metabolic pathways in plants established the importance of 14-3-3 proteins. In the present study, a total of 22 genes, also called general regulatory factors ( ), were identified in the peanut ( ) genome, out of which 12 belonged to the ε group, whereas 10 of them belonged to the non- ε-group. Tissue-specific expression of identified genes were studied using transcriptome analysis. The peanut gene was cloned and transformed into . The investigation of subcellular localization indicated that is localized in the cytoplasm. Overexpression of the gene in transgenic showed that under exogenous 1-naphthaleneacetic acid (NAA) treatment, root growth inhibition in transgenic plants was enhanced. Further analysis indicated that the expression of auxin-responsive genes , , , and was upregulated and and were downregulated in transgenic plants, but the expression of , , and showed opposite trends of change under NAA treatment. These results suggest that may be involved in auxin signaling during seedling root development. An in-depth study of the molecular mechanism of this process remains to be further explored.