Freshwater microbial metagenomes sampled across different water body characteristics, space and time in Israel

Freshwater bodies are critical components of terrestrial ecosystems. The microbial communities of freshwater ecosystems are intimately linked water quality. These microbes interact with, utilize and recycle inorganic elements and organic matter. Here, we present three metagenomic sequence datasets (total of 182.9 Gbp) from different freshwater environments in Israel. The first dataset is from diverse freshwater bodies intended for different usages – a nature reserve, irrigation and aquaculture facilities, a tertiary wastewater treatment plant and a desert rainfall reservoir. The second represents a two-year time-series, collected during 2013–2014 at roughly monthly intervals, from a water reservoir connected to an aquaculture facility. The third is from several time-points during the winter and spring of 2015 in Lake Kinneret, including a bloom of the cyanobacterium Microcystis sp. These datasets are accompanied by physical, chemical, and biological measurements at each sampling point. We expect that these metagenomes will facilitate a wide range of comparative studies that seek to illuminate new aspects of freshwater microbial ecosystems and inform future water quality management approaches. Measurement(s) pH measurement • temperature of water • concentration of oxygen in water • total phosphorus • ammonia • concentration of nitrate in water • concentration of nitrite in water • concentration of chlorophyll a in water • concentration of chlorophyll b in water • concentration of beta-carotene in water • concentration of fucoxanthin in water • Dinoxanthin • Diadinoxanthin • Diatoxanthin • concentration of zeaxanthin in water • concentration of lutein in water • microcystin-LR • library DNA concentration Technology Type(s) pH Meter • Temperature Probe Device • electron spin resonance oximetry • colorimetry • colorimetry • liquid chromatography-photodiode array detection • liquid chromatography-photodiode array detection • microcystins/Nodularins ADDA Elisa • PicoGreen Dye Factor Type(s) Geographic location • Isolaion source • Depth • latitude • longitude Sample Characteristic - Environment freshwater biome Sample Characteristic - Location Israel