Are Hamsters a Suitable Model for Evaluating the Immunogenicity of RBD-Based Anti-COVID-19 Subunit Vaccines?

Currently, SARS-CoV-2 spike receptor-binding-domain (RBD)-based vaccines are considered one of the most effective weapons against COVID-19. During the first step of assessing vaccine immunogenicity, a mouse model is often used. In this paper, we tested the use of five experimental animals (mice, ham...

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Veröffentlicht in:	Viruses 2022-05, Vol.14 (5), p.1060
Hauptverfasser:	Merkuleva, Iuliia A, Shcherbakov, Dmitry N, Borgoyakova, Mariya B, Isaeva, Anastasiya A, Nesmeyanova, Valentina S, Volkova, Natalia V, Aripov, Vazirbek S, Shanshin, Daniil V, Karpenko, Larisa I, Belenkaya, Svetlana V, Kazachinskaia, Elena I, Volosnikova, Ekaterina A, Esina, Tatiana I, Sergeev, Alexandr A, Titova, Kseniia A, Konyakhina, Yulia V, Zaykovskaya, Anna V, Pyankov, Oleg V, Kolosova, Evgeniia A, Viktorina, Olesya E, Shelemba, Arseniya A, Rudometov, Andrey P, Ilyichev, Alexander A
Format:	Artikel
Sprache:	eng
Schlagworte:	animal models Animals Antibodies Bioethics Chickens Cloning Coronaviruses COVID-19 COVID-19 - prevention & control COVID-19 vaccines COVID-19 Vaccines - immunology Cricetinae Disease Models, Animal Disease transmission Eggs Enzyme-linked immunosorbent assay Epidemics Ferrets Immune response (humoral) Immunogenicity Immunogenicity, Vaccine Infections Laboratories Mice Mice, Inbred BALB C Proteins Rabbits receptor-binding domain Respiratory diseases SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Spike Glycoprotein, Coronavirus - immunology Vaccines Vaccines, Subunit - immunology
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Zusammenfassung:	Currently, SARS-CoV-2 spike receptor-binding-domain (RBD)-based vaccines are considered one of the most effective weapons against COVID-19. During the first step of assessing vaccine immunogenicity, a mouse model is often used. In this paper, we tested the use of five experimental animals (mice, hamsters, rabbits, ferrets, and chickens) for RBD immunogenicity assessments. The humoral immune response was evaluated by ELISA and virus-neutralization assays. The data obtained show hamsters to be the least suitable candidates for RBD immunogenicity testing and, hence, assessing the protective efficacy of RBD-based vaccines.
ISSN:	1999-4915 1999-4915
DOI:	10.3390/v14051060