Análise microbiológica da água utilizada para consumo nas escolas de Esperança, Paraíba

This project had the objective of verifying the microbiological quality of the water that supplies seven public schools in the urban area of the municipality of Esperança-PB. Initially, the samples were collected in wide - mouth glass bottles, protected with laminated paper, pre-sterilized (121º C - 30’ in autoclave), and were sent to the Environmental Sanitation Laboratory of the State University of Paraíba - Campina Grande Campus, where the qualitative analysis of Total Coliforms and Escherichia coli were performed using the Colilert Enzymatic Chromogenic Substrate method. Soon after the application of the Colilert reagent and incubation for 24 hours at 37 ° C, a yellow coloration was observed in samples 6 and 7, highlighting the presence of total coliforms. However, the presence of E. coli was not detected in any of the analyzed samples, since they did not show fluorescence under ultraviolet light. This concludes that the waters of samples 6 and 7 are inappropriate for human consumption. According to Ordinance No. 2.914 / 2011 of the Ministry of Health, when samples with positive results are detected for total coliforms, even in presumptive tests, corrective actions must be taken and new samples must be collected on successive days until satisfactory results are obtained.