Effect of Xingbi Gel Nasal Drops on Rats with Allergic Rhinitis Based on the Nasal Mucosa-Hippocampus Neuroimmune Mechanism

ObjectiveTo explore the effect of Xingbi gel nasal drops on rats with allergic rhinitis (AR) based on the nasal mucosa-hippocampus neuroimmune mechanism.MethodsA total of 40 SPF SD rats at postnatal day 21 (PND21) were randomly divided into normal group, model group, traditional Chinese medicine group and western medicine group, with 10 cases in each group. Except for the normal group, the other three groups were sensitized by ovalbumin (OVA) to establish AR animal models. After the model was successfully established, the normal group and model group were both given normal saline nasal drops (50 μL once), the traditional Chinese medicine group was given Xingbi gel nasal drops (50 μL once), and the western medicine group was given budesonide nasal spray (20 μL once), twice a day, and the interventions were continued for 12 days. The AR rat behavioral scoring criteria was used to evaluate rhinitis behavior of rats; Morris water maze test was used to evaluate learning and memory function of rats; enzyme-linked immunosorbent assay (ELISA) was used to detect serum levels of immunoglobulin E (IgE) and transforming growth factor-β1 (TGF-β1); hematoxylin-eosin (HE) staining was used to observe pathological changes of nasal mucosa and eosinophil (EOS) count; immunohistochemistry method was used to detect expression levels of non-receptor tyrosine kinases Fyn, substance P (SP), vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) in the hippocampus; and real-time polymerase chain reaction (RT-PCR) was used to detect mRNA transcription levels of Fyn, SP, VIP and NPY in the hippocampus.Results(1) Behavioral scores of rhinitis: compared with the normal group, behavioral scores of rhinitis were significantly higher in the model group, the traditional Chinese medicine group and the western medicine group before treatment (P