Injectable and in situ foaming shape-adaptive porous Bio-based polyurethane scaffold used for cartilage regeneration

Irregular articular cartilage injury is a common type of joint trauma, often resulting from intense impacts and other factors that lead to irregularly shaped wounds, the limited regenerative capacity of cartilage and the mismatched shape of the scaffods have contributed to unsatisfactory therapeutic outcomes. While injectable materials are a traditional solution to adapt to irregular cartilage defects, they have limitations, and injectable materials often lack the porous microstructures favorable for the rapid proliferation of cartilage cells. In this study, an injectable porous polyurethane scaffold named PU-BDO-Gelatin-Foam (PUBGF) was prepared. After injection into cartilage defects, PUBGF forms in situ at the site of the defect and exhibits a dynamic microstructure during the initial two weeks. This dynamic microstructure endows the scaffold with the ability to retain substances within its interior, thereby enhancing its capacity to promote chondrogenesis. Furthermore, the chondral repair efficacy of PUBGF was validated by directly injecting it into rat articular cartilage injury sites. The injectable PUBGF scaffold demonstrates a superior potential for promoting the repair of cartilage defects when compared to traditional porous polyurethane scaffolds. The substance retention ability of this injectable porous scaffold makes it a promising option for clinical applications. [Display omitted] •The injectable PUBGF can be administered via injection to fill wounds of any shape within cartilage defects, and subsequently takes shape in situ, forming a porous scaffold that exhibits commendable mechanical properties and high porosity. The injectable PUBGF, upon in vivo formation, exhibits a dynamic microarchitecture conducive to the retention of substances, thereby further enhancing chondrogenic differentiation and demonstrating a markedly improved efficacy in cartilage repair.