Construction of Thermo-Responsive Elastin-Like Polypeptides (ELPs)-Aggregation-Induced-Emission (AIE) Conjugates for Temperature Sensing

In this work, an aggregation-induced emission (AIE) molecule (tetraphenylethene derivative, TPE-COOH) was conjugated to elastin-like polypeptides (ELPs40) via an amide bond to form ELPs40-TPE. The successful synthesis of ELPs40-TPE was confirmed by Circular Dichroism spectroscopy, gel electrophoresi...

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Veröffentlicht in:	Molecules (Basel, Switzerland) Switzerland), 2018-07, Vol.23 (7), p.1725
Hauptverfasser:	Chen, Zhe, Ding, Zhaoyang, Zhang, Guangya, Tian, Leilei, Zhang, Xuanjun
Format:	Artikel
Sprache:	eng
Schlagworte:	aggregation-induced-emission (AIE) Biocompatibility Circular dichroism Dichroism Elastin Elastin - chemical synthesis Elastin - chemistry Elastin - pharmacology elastin-like polypeptides (ELPs) Electrophoresis Emission spectroscopy Emissions Fluorescence Gel electrophoresis HeLa Cells Humans Polypeptides Spectroscopy Spectrum analysis Temperature Temperature dependence temperature sensing thermo-responsive
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Beschreibung
Zusammenfassung:	In this work, an aggregation-induced emission (AIE) molecule (tetraphenylethene derivative, TPE-COOH) was conjugated to elastin-like polypeptides (ELPs40) via an amide bond to form ELPs40-TPE. The successful synthesis of ELPs40-TPE was confirmed by Circular Dichroism spectroscopy, gel electrophoresis, UV-vis absorption, and fluorescence emission spectroscopy. ELPs40-TPE possessed both amphiphilicity and the features of an AIE, and the fluorescence intensity was dependent on the local temperature. The Hela cells imaging indicated that ELPs40-TPE has great potential for bio-imaging applications because of its advantages of high fluorescence intensity, good water-solubility, and remarkable biocompatibility.
ISSN:	1420-3049 1420-3049
DOI:	10.3390/molecules23071725