Plasma small‐extracellular vesicles enriched in miR‐122‐5p promote disease aggressiveness in pediatric anaplastic large‐cell lymphoma

Abbreviations ALCL Anaplastic Large Cell Lymphoma ALDO A Aldolase A ALK+ Anaplastic Lymphoma Kinase positive AML12 Alpha Mouse Liver 12 CD30 Cluster of Differentiation 30 CM Conditioned Media CS Citrate Synthase ECAR Extracellular Acidification Rate EFS Event Free Survival GFP Green Fluorescent Protein GLUT1 Glucose Transporter 1 HD Healthy Donors MEF-1 Mouse Embryonic Fibroblasts-1 miR-122-5p microRNA-122-5p miRNA microRNA NC Negative Control NPM Nucleophosmin PCA Principal Component Analysis PKM2 Pyruvate Kinase M2 RT-qPCR Real-Time quantitative-PCR sRNA-seq Small RNA-sequencing analysis S-EVs Small Extracellular Vesicles Dear Editor, Anaplastic lymphoma kinase-positive (ALK+) anaplastic large cell lymphoma (ALCL) is an aggressive peripheral T-cell lymphoma representing 10%-15% of pediatric lymphoid neoplasms. [...]in a panel of ALCL cell lines, miR-122-5p was not expressed, while alpha mouse liver 12 (AML12) cells and S-EVs showed high miR-122-5p levels (Supplementary Figure S1D). Interestingly, we observed significantly higher transaminase values at diagnosis in ALCL patients with miR-122-5p levels above the median value (Supplementary Figure S1F). [...]we hypothesized that the increased circulating levels of miR-122-5p might result from disease-related liver function impairment in patients with advanced ALCL. Principal Component Analysis; PKM2: Pyruvate Kinase M2; RNA-seq: RNA-sequencing analysis; RT-qPCR: Real Time-quantitative PCR; S-EVs: Small Extracellular Vesicles; SEM: