Effects of two different doses of zinc sulfate on serum troponin I 3 enzyme level and cardiac malondialdehyde contents in mitoxantrone-induced cardiotoxicity in rats

Mitoxantrone antineoplastic drugs used in the treatment cancers. The mitoxantrone' may cause cardiotoxicity which is irreversible and dose-dependent. Zinc is regarded as a necessary element for cell division and the production of DNA and protein; furthermore, it has a main role in alleviating cardiovascular diseases; and may have protective outcomes in coronary artery disease. The current research is designed to investigate the effects of two different doses of zinc sulfate on mitoxantrone-induced cardiotoxicity in rats. Forty-eight (48) adult Albino rats of both sexes were utilized in this study; the animals were randomly classified into six groups of 8 animals each. Group I: Rats were received distilled water (negative control). Group II: Orally-administered zinc sulfate (15mg/kg/day) Group III: orally-administered zinc sulfate (30mg/kg/day) Group IV: Rats Intraperitoneally injected with a mitoxantrone at a dose of (2. 5 mg/kg) to reach total cumulative dose of 7. 5 mg/kg on day 20 (positive control). Group V: Orally-administered zinc sulfate at a dose (15mg/kg/day) and an intraperitoneal injection of mitoxantrone at a dose (2. 5 mg/kg) was administered to reach the total cumulative dose of 7. 5 mg/kg on day 20. Group VI: Orally-administered zinc sulfate at a dose (30 mg/kg/day), and an intraperitoneal injection of mitoxantrone at a dose (2. 5 mg/kg) to reach the total cumulative dose of 7. 5 mg/kg on day 20. Forty-eight (48) hours after the end of treatment duration (i. e. at day 22 nd), each animal was euthanized by diethyl ether and ketamine. Then, after cervical dislocation, blood was obtained by cardiac puncture and then serum was prepared to estimate troponin I 3 enzyme levels, and the heart of each animal was excised for homogenate preparation to estimate of malondialdehyde contents. It was found that oral administration of zinc sulfate [(15mg/kg/day with total cumulative dose (7. 5 mg/kg) of MTXN] (Group V), resulted in a non-significant (P>0. 05) differences in both serum level of troponin I 3 enzyme, and malondialdehyde (MDA) contents in heart tissue homogenate compared to the corresponding level and content in group of rats IP injected with total cumulative dose of 7. 5 mg/kg of MTXN (Group IV). Moreover, there were significant reductions (P