Host-microbiome interactions in response to a high-saturated fat diet and fish-oil supplementation in zebrafish adult

[Display omitted] •The consumption of a high-fat-saturated diet induces changes in body lipid profile of zebrafish adults and on the intestinal microbiota.•The supplementation of the high-fat-saturated diet with commercial fish oil, rich in DHA, alleviates both lipid profile and microbial changes. Diet is one of the main factors affecting host’s health. The aim of this work was to study the interaction among nutrition, microbiota and host, using zebrafish adults as animal model. Thus, the effects of a high-saturated-fat diet, and its supplementation with a commercial fish-oil on fish lipid profile, intestinal microbiota and blood glucose were evaluated. The dietary saturated fat changed the fish lipid profile, microbial community composition, and its metabolism. Saturated fatty acids levels were higher in fish fed the high-saturated-fat diet, which correlated with an increased in Pseudomonas. Otherwise, the commercial fish-oil intake ameliorated the effect of the fat on the lipid profile, lowering saturated fatty acid levels while increasing polyunsaturated fatty acids. It also contributed to limit the growth of Pseudomonas on intestinal microbial community. Furthermore, blood glucose diminished in animals fed fish-oil supplemented diet. This suggests that fish-oil may mitigate the effect of the high-saturated-fat-diet.