Estimating the inhibitory effect of Lactobacillus isolated from different sources on some pathogens of urogenital infections in women group

This study was aimed to identify of pathogenic organism isolated from urogintal tract and estimate the effect of Lactobacillus which isolated from different sources on the growth of these pathogens including (Candida albicans, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Gardnerella viginals, Klebsiella oxytoca ,Proteus mirabilis). The results showed that Lactobacillus which was isolated from vagina on solid medium was effective against pathogenic isolates more than the Lactobacillus species isolated from yoghurt, cow milk, human milk with inhibition zone (12-16) mm, while the highs inhibitory effect of Lactobacillus isolated from vagina in liquid media with inhibition zone reached to(18)mm. Adversely, lowest inhibitory effect was shown with supernatant of Lactobacillus spp isolated from human milk with inhibition zone reached to(11)mm comparison with Lactobacillus spp isolated from yoghurt and cow milk with inhibition zone (13-15) mm. Also the result revealed that Lactobacillus spp isolated from vagina, human milk, cow milk and yoghurt by overlay method had no effect on pathogenic bacteria but high effect was shown only with the vaginal Lactobacillus isolates on C .albicans.