Lactobacillus acidophilus NCFM and Lactiplantibacillus plantarum Lp-115 inhibit Helicobacter pylori colonization and gastric inflammation in a murine model

To determine the role of Lactobacillus strains and their combinations in inhibiting the colonization of and gastric mucosa inflammation. Human gastric adenocarcinoma AGS cells were incubated with H. pylori and six probiotic strains ( NCFM, La-14, Lp-115, Lpc-37, Lr-32, and GG) and the adhesion ability of in different combinations was evaluated by fluorescence microscopy and urease activity assay. Male C57BL/6 mice were randomly divided into five groups (uninfected, , +NCFM, +Lp-115, and +NCFM+Lp-115) and treated with two lactobacilli strains (NCFM and Lp-115) for six weeks. colonization and tissue inflammation statuses were determined by rapid urease test, Hematoxylin-Eosin (HE) staining, immunohistochemistry, and qRT-PCR and ELISA. NCFM, La-14, Lp-115, Lpc-37, Lr-32, and GG reduced adhesion and inflammation caused by infection in AGS cells and mice. Among all probiotics NCFM and , Lp-115 showed significant effects on the eradication and reduction of inflammation and . Compared with the infection group, the mRNA and protein expression levels of IL-8 and TNF-α in the six intervention groups were significantly reduced. The changes in the urease activity ( and ) for 1-7h in each group showed that NCFM, La-14, Lp-115, and GG effectively reduced the colonization of . We observed a higher ratio of lymphocyte and plasma cell infiltration into the lamina propria of the gastric mucosa and neutrophil infiltration in +NCFM+Lp-115 mice. The infiltration of inflammatory cells in lamina propria of the gastric mucosa was reduced in the +NCFM+Lp-115 group. Additionally, the expression of IFN-γ was decreased significantly in the NCFM and Lp-115 treated C57BL/6 mice. NCFM and Lp-115 can reduce the adhesion of and inhibit the gastric inflammatory response caused by infection.