Spatial Ecology of the Human Tongue Dorsum Microbiome

A fundamental question in microbial ecology is how microbes are spatially organized with respect to each other and their host. A test bed for examining this question is the tongue dorsum, which harbors a complex and important microbial community. Here, we use multiplexed fluorescence spectral imaging to investigate the organization of the tongue microbiome at micron to hundred-micron scales. We design oligonucleotide probes for taxa both abundant and prevalent, as determined by sequence analysis. Imaging reveals a highly structured spatial organization of microbial consortia, ranging in linear dimension from tens to hundreds of microns. The consortia appear to develop from a core of epithelial cells, with taxa clustering in domains suggestive of clonal expansion. Quantitative proximity analysis provides the basis for a model of tongue dorsum microbiome organization and dynamics. Our work illustrates how high-resolution analysis of micron-scale organization provides insights into physiological functions and microbiome-host interactions. [Display omitted] •Bacteria on the tongue form large organized consortia with a patch mosaic structure•Consortia are organized around a core of keratinized epithelial cells•Spatial organization can be quantified and permits inferences on dynamics How microbes are spatially organized is a fundamental question in microbial ecology about which little is known. Using fluorescence spectral imaging, Wilbert et al. discover dense, highly structured microbial consortia on the human tongue. Analysis of the organization of these consortia permits inferences about community dynamics and oral microbial ecology.