Real-Time Monitoring of Stem Cells by Diamond-Based Impedance Sensors

Real-Time Monitoring of Stem Cells by Diamond-Based Impedance Sensors

Cell-based impedance spectroscopy is a promising label-free method for electrical monitoring of cell activity. Here we present a diamond-based impedance sensor with built-in gold interdigitated electrodes (IDEs) as a promising platform for simultaneous electrical and optical monitoring of adipose-de...

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Veröffentlicht in:Proceedings 2017-08, Vol.1 (4), p.515
Hauptverfasser: Václav Procházka, Roman Matějka, Tibor Ižák, Ondrej Szabó, Jana Štěpanovská, Lucie Bačáková, Alexander Kromka
Format: Artikel
Sprache:eng
Schlagworte:
biosensor
diamond film
impedance spectroscopy
stem cells
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Zusammenfassung:Cell-based impedance spectroscopy is a promising label-free method for electrical monitoring of cell activity. Here we present a diamond-based impedance sensor with built-in gold interdigitated electrodes (IDEs) as a promising platform for simultaneous electrical and optical monitoring of adipose-derived stem cells (ASC). The impedance spectra were collected in a wide frequency range (from 100 Hz to 50 kHz) for 27 h of cultivation in chambers designed for static cultivation. Absolute impedance spectra were analyzed in terms of measurement frequencies and cell properties monitored by high-resolution digital camera.
ISSN:2504-3900
DOI:10.3390/proceedings1040515