Antimicrobial potential of lactic acid bacteria from domestic chickens (Gallus domesticus) from south Celebes, Indonesia, in different growth phases: in vitro experiments supported by computational docking

Pathogenic bacterial infection is one of the factors that can cause extensive losses in poultry farming. Pathogenic bacteria that infect domestic chickens include . This study has investigated antimicrobial compounds from probiotic bacteria isolated from the digestive tract of domestic chickens originating from Takalar Regency, South Sulawesi, Indonesia. Lactic acid bacteria were grown on de Man-Ragosa-Sharpe agar medium for 24 hours. The bacterial isolate with the best inhibitory power was identified as , based on 16S RNA sequences. Antimicrobial activity of the selected lactic acid bacteria was tested on the pathogenic bacteria, and . Using well diffusion method. In this study, in silico study was conducted to examine the structure and binding affinity of lactic acid bacteria against and . Molecular docking experiments were performed using the PyRx 0.8 software. This study showed that the bacteria were strain PATA-5. The response of inhibition of antimicrobial compounds produced by strain PATA-5 maximum in the stationary phase. The bactericidal properties of strain PATA-5 were categorized as strong against Gram-negative , i.e., 30.5 mm, when compared to Gram-positive , i.e., 17.5 mm. strain PATA-5 is capable to produce natural antibiotic cyclic lipopeptides, namely surfactin.