Enrichment and Detection of Antigen-Binding B Cells for Mass Cytometry

Enrichment and Detection of Antigen-Binding B Cells for Mass Cytometry

Over the years, various techniques have been utilized to study the function and phenotype of antigen-binding B cells in the primary repertoire following immunization, infection, and development of autoimmunity. Due to the low frequency of antigen-reactive B cells (

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Bibliographische Detailangaben
Veröffentlicht in:Magnetochemistry 2021-07, Vol.7 (7), p.92
Hauptverfasser: Stensland, Zachary C, Smith, Mia J
Format: Artikel
Sprache:eng
Schlagworte:
Antibodies
antigen-binding B cells
antigen-reactive B cells
antigen-specific B cells
Antigens
Binding
Contaminants
CyTOF
Enrichment
Flow cytometry
Immunization
Insulin
Lymphocytes
magnetic enrichment
mass cytometry
Mass spectrometry
Nanoparticles
Scientific imaging
Statistical methods
Tetanus
Vortices
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Beschreibung
Zusammenfassung:Over the years, various techniques have been utilized to study the function and phenotype of antigen-binding B cells in the primary repertoire following immunization, infection, and development of autoimmunity. Due to the low frequency of antigen-reactive B cells (
ISSN:2312-7481
2312-7481
DOI:10.3390/magnetochemistry7070092