Fine-tuning sugar content in strawberry

Fine-tuning sugar content in strawberry

Fine-tuning quantitative traits for continuous subtle phenotypes is highly advantageous. We engineer the highly conserved upstream open reading frame (uORF) of FvebZIPs1.1 in strawberry (Fragaria vesca), using base editor A3A-PBE. Seven novel alleles are generated. Sugar content of the homozygous T1...

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Veröffentlicht in:Genome Biology 2020-09, Vol.21 (1), p.230-230, Article 230
Hauptverfasser: Xing, Sinian, Chen, Kunling, Zhu, Haocheng, Zhang, Rui, Zhang, Huawei, Li, Bingbing, Gao, Caixia
Format: Artikel
Sprache:eng
Schlagworte:
Alleles
Amino acids
Asexual reproduction
Asexuality
Asexually reproducing crops
Basic leucine zipper
Carbohydrate Metabolism
DNA binding proteins
Fine-tuning
Fragaria - genetics
Fragaria - metabolism
Fragaria vesca
Fruit - metabolism
Gene Editing
Gene expression
Genetic aspects
Genome editing
Genomes
Genomics
Genotypes
Metabolism
Method
Mutants
Mutation
Open Reading Frames
Peptides
Phenotypes
Quantitative trait variation
Quantitative Trait, Heritable
Reproduction, Asexual
Strawberry
Sucrose
Sugar
Transcription factors
Transgenic plants
Upstream open reading frame
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Zusammenfassung:Fine-tuning quantitative traits for continuous subtle phenotypes is highly advantageous. We engineer the highly conserved upstream open reading frame (uORF) of FvebZIPs1.1 in strawberry (Fragaria vesca), using base editor A3A-PBE. Seven novel alleles are generated. Sugar content of the homozygous T1 mutant lines is 33.9-83.6% higher than that of the wild-type. We also recover a series of transgene-free mutants with 35 novel genotypes containing a continuum of sugar content. All the novel genotypes could be immediately fixed in subsequent generations by asexual reproduction. Genome editing coupled with asexual reproduction offers tremendous opportunities for quantitative trait improvement.
ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-020-02146-5