Evaluation of the genetic diversity among maize lines (Zea mays L.) by DNA fingerprinting analysis Avaliação de diversidade genética entre linhagens de milho (Zea mays L.) por analisis de "DNA fingerprinting

Informativo DNA fingerprint profiles of eight homozygotic maize lines were obtained by the electrophoretic separation of DNA restriction fragments and the ir hybridization with the minisatellite probe R18.1. The analysis of the bandsharing frequencies allowed to identify all the lines and to estimat...

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Veröffentlicht in:Ciência rural 1996-12, Vol.26 (3), p.501-503
Hauptverfasser: Sergio Echeverrigaray, Ana Paula Longaray Delamare, Geraldo Tosello, Orit Gal, Jossi Hillel, Uri Lavi
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Sprache:eng
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Zusammenfassung:Informativo DNA fingerprint profiles of eight homozygotic maize lines were obtained by the electrophoretic separation of DNA restriction fragments and the ir hybridization with the minisatellite probe R18.1. The analysis of the bandsharing frequencies allowed to identify all the lines and to estimate the genetic distances between them. The relationship obtained by DNA fingerprinting analysis of the eight inbreed lines was highly consistem with the ir genetical origin.Perfis altamente informativos de oito linhagens homozigotas de milho foram obtidos através de análise de DNA fingerprinting, hibridizando-se os fragmentos de restrição com a sonda minisatélite R18,1. A análise de bandas coincidentes permitiu identificar todas as linhagens e estimar as distâncias genéticas entre elas. A relação entre as linhagens obtidas por esta análise é consistente com a origem genética das mesmas.
ISSN:0103-8478
1678-4596
DOI:10.1590/S0103-84781996000300028