Sequencing and analysis of the complete mitochondrial genome of Rugosa emeljanovi (Anura: Ranidae)

In this study, the complete mitogenome sequence of Rugosa emeljanovi (Anura: Ranidae) is first determined using long PCR. It is a circular molecule of 17,733 bp in length (GenBank accession no. KU641020). Similar to the typical mtDNA of amphibians, the complete mtDNA sequence of R. emeljanovi contained 2 rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, 13 protein-coding genes (PCGs), and a control region (D-loop). The nucleotide composition was 28.1% A, 26.8% C, 14.8% G, and 30.3% T. Mitochondrial genome analyses based on the NJ method yield phylogenetic trees, including the 13 already reported Ranidae family frogs. The molecular data presented here provide a useful tool for systematic analyses of genus Rugosa and Glandirana.