In vitro anticoagulant effect of Crassocephalum crepidioides leaf methanol extract and fractions on human blood

Blood coagulation is a rapid and efficient process that produces clot formation which requires regulation. A derangement of blood coagulation is a feature of many disease conditions. This study investigated the in vitro effects of Benth S. Moore leaf methanol (crude) extract and its partitioned solvent fractions on blood coagulation of Healthy human volunteers. The secondary metabolites from dried and ground leaves were extracted with 70% methanol, and the concentrated crude extract was subsequently subjected to solvent partitioning with Hexane, Ethyl acetate, and Butanol. Varying concentrations (5-20 mg/mL) of the extract and fractions were tested in vitro on blood coagulation profile; clotting time (CT), prothrombin time (PT), and activated partial thromboplastin time (aPTT) of apparently healthy human volunteers, while phytochemical characterization of the Hexane fraction was done by gas chromatography-mass spectrometry (GC-MS). leaf methanol extract and fractions significantly (