Effect of post-exercise lactate administration on glycogen repletion and signaling activation in different types of mouse skeletal muscle

Lactate is not merely a metabolic intermediate that serves as an oxidizable and glyconeogenic substrate, but it is also a potential signaling molecule. The objectives of this study were to investigate whether lactate administration enhances post-exercise glycogen repletion in association with cellular signaling activation in different types of skeletal muscle. Eight-week-old male ICR mice performed treadmill running (20 m/min for 60 min) following overnight fasting (16 h). Immediately after the exercise, animals received an intraperitoneal injection of phosphate-buffered saline or sodium lactate (equivalent to 1 g/kg body weight), followed by oral ingestion of water or glucose (2 g/kg body weight). At 60 min of recovery, glucose ingestion enhanced glycogen content in the soleus, plantaris, and gastrocnemius muscles. In addition, lactate injection additively increased glycogen content in the plantaris and gastrocnemius muscles, but not in the soleus muscle. Nevertheless, lactate administration did not significantly alter protein levels related to glucose uptake and oxidation in the plantaris muscle, but enhanced phosphorylation of TBC1D1, a distal protein regulating GLUT4 translocation, was observed in the soleus muscle. Muscle FBP2 protein content was significantly higher in the plantaris and gastrocnemius muscles than in the soleus muscle, whereas MCT1 protein content was significantly higher in the soleus muscle than in the plantaris and gastrocnemius muscles. The current findings suggest that an elevated blood lactate concentration and post-exercise glucose ingestion additively enhance glycogen recovery in glycolytic phenotype muscles. This appears to be associated with glyconeogenic protein content, but not with enhanced glucose uptake, attenuated glucose oxidation, or lactate transport protein. [Display omitted] •Lactate is an oxidizable and glyconeogenic metabolic intermediate and signaling molecule.•Lactate injection and glucose ingestion additively enhanced post-exercise glycogen recovery in plantaris and gastrocnemius, but not soleus muscles.•Lactate injection did not change glucose uptake and oxidation in plantaris muscle, but it enhanced phosphorylation of TBC1D1 in soleus muscle.•Muscle protein isotype FBP2 content was significantly higher in plantaris and gastrocnemius muscles than in soleus muscle.•The current findings suggest that elevated circulating lactate and carbohydrate consumption additively enhances post-exercise glycogen recovery in