Correlation of NRF2 and progesterone receptor and its effects on ovarian cancer biology
This study aimed to investigate the potential prognostic impact of nuclear factor erythroid 2-related factor 2 (NRF2) and progesterone receptor A (PRA)/progesterone receptor B (PRB) in ovarian cancer patients which might be the rationale for putative new treatment strategies. The presence of NRF2 an...
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Veröffentlicht in: | Cancer management and research 2019-08, Vol.11, p.7673-7684 |
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Sprache: | eng |
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Zusammenfassung: | This study aimed to investigate the potential prognostic impact of nuclear factor erythroid 2-related factor 2 (NRF2) and progesterone receptor A (PRA)/progesterone receptor B (PRB) in ovarian cancer patients which might be the rationale for putative new treatment strategies.
The presence of NRF2 and PRA/PRB was investigated in 156 ovarian cancer samples using immunohistochemistry (IHC). Staining of NRF2 and PRA/PRB was rated using the semi-quantitative immunoreactive score (IR score, Remmele's score) and correlated to clinical and pathological data. NRF2 and PRA/PRB expression were compared with respect to the overall survival (OS).
NRF2 staining was different in both, the cytoplasm and nucleus between the histological subtypes (
=0.001 and
=0.02, respectively). There was a significant difference in the PRA expression comparing all histological subtypes (
=0.02). Histological subtypes showed no significant differences in the PRB expression. A strong correlation of cytoplasmic NRF2 and PRA expression was detected (cc=0.247,
=0.003) as well as of cytoplasmic NRF2 and PRB expression (cc=0.25,
=0.003), confirmed by immunofluorescence double staining. Cytoplasmic NRF2 expression was associated with a longer OS (median 50.6 vs 32.5 months;
=0.1) as it was seen for PRA expression (median 63.4 vs 33.1 months;
=0.08), although not statistically significant. In addition, high PRB expression (median 80.4 vs 32.5 months;
=0.04) and concurrent expression of cytoplasmic NRF2 and PRA were associated with a significantly longer OS (median 109.7 vs 30.6 months;
=0.02). The same relationship was also noted for NRF2 and PRB with improved OS for patients expressing both cytoplasmic NRF2 and PRB (median 153.5 vs 30.6 months;
=0.009). Silencing of
induced higher mRNA expression of
in the cancer cell line OVCAR3 (
>0.05) confirming genetic interactions of NRF2 and PR.
In this study, the combination of cytoplasmic NRF2 and high PRA/PRB expression was demonstrated to be associated with improved overall survival in ovarian cancer patients. Further understanding of interactions within the NRF2/AKR1C1/PR pathway could open new additional therapeutic approaches. |
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ISSN: | 1179-1322 1179-1322 |
DOI: | 10.2147/CMAR.S210004 |