PRB expression in stomach of neonate canine without establish breed

The cells of the gastrointestinal epithelial mucosa are constantly under high regenerative pressure, due to the expeditious rate of cell turnover in this tissue. To regulate homeostasis in the gastrointestinal tract, a vital balance between cell apoptosis, senescence and the proliferation and differentiation of new cells must be maintained. The present research aimed was analyse the performance of the retinoblastoma protein in different parts of the stomach from neonate dogs. Were used forty five canine neonates, obtained at private veterinary clinics and Veterinary Hospital of Federal University of Piauí. The animals were from females with dystocia or pregnant, at the end of gestational stage, who had problems during parturition and/or cesarean section whose puppies had died. From these animals were obtained stomach fragments (about 0.5cm) that were fixed in buffered formaldehyde and submitted to histological routine, stained with hematoxylin and eosin, for light microscopy. Then the best blocks were submitted a immunohistochemistry process, using anti-body pRb. The data presented in this paper, strongly suggest that the Rb protein is coupled with apoptosis directly related to the number of cells and renewal of epithelial regulation and gastric mucosa.