A study on the changes in rice composition under reduced fertilization conditions using Raman spectroscopy technology

The substitution of microbial fertilizer for chemical fertilizer can not only improve soil fertility but also effectively enhance rice quality. To investigate the effect of different amounts of combined application of chemical fertilizer and microbial fertilizer on the amylose content of rice, this study adopts theoretical calculations to compare the preprocessed Raman spectroscopy information of rice with reduced fertilization and establishes a recognition model for the amylose content of rice, which is used to detect the amylose content in rice. Based on the amylose spectral values measured by Raman spectroscopy and the known starch structure and functional groups, the Raman peaks are mainly distributed in the range of 400 cm − 1 to 1400 cm − 1 . The Raman characteristic peaks at 483 cm − 1 , 869 cm − 1 , 933 cm − 1 , 1082 cm − 1 , 1126 cm − 1 , 1335 cm − 1 , 1385 cm − 1 , and 1455 cm − 1 exhibit strong vibration modes, which are consistent with its main nutrient component of amylose. By comparing the measured amylose content in the regions treated with microbial fertilizer combined with different amounts of reduced fertilization and the spectral intensity values of amylose measured by Raman spectroscopy, the results show that the treatment of combining conventional amounts of microbial fertilizer with different amounts of reduced chemical fertilizer exhibits a decreasing trend in the amylose content of rice.