Functional study and regional mapping of 44 hormono-regulated genes isolated from a porcine granulosa cell library

cDNA clones from a pig granulosa cell cDNA library were isolated by differential hybridisation for follicle stimulating hormone (FSH) regulation in granulosa cells in a previous study. The clones that did not match any known sequence were studied for their expression in granulosa cells (treated or n...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Genetics selection evolution (Paris) 2001-01, Vol.33 (1), p.69-87, Article 69
Hauptverfasser:	Tosser Klopp, G. (Institut National de la Recherche Agronomique, Castanet Tolosan (France). Centre de Toulouse, Laboratoire de Genetique Cellulaire), Bonnet, A, Yerle, M, Hatey, F
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN Animal productions Animals Biological and medical sciences Biotechnology Blotting, Northern CARTE GENETIQUE cDNA mapping Cells Cells, Cultured CERDO Chromosome Mapping comparative map comparative RT-PCR Cytogenetics DNA Expressed Sequence Tags Female FOLICULOS OVARICOS Follicle Stimulating Hormone - pharmacology Follicle-stimulating hormone Follicles FOLLICULE OVARIEN Fundamental and applied biological sciences. Psychology GENE Gene Expression Regulation Gene Library Gene mapping GENES Genetic aspects GENETIC MAPS Genetics Genetics of eukaryotes. Biological and molecular evolution Genome Genomes Granulosa cells Granulosa Cells - drug effects HORMONAS HORMONE Hormone metabolism and regulation HORMONES Humans Hybridization Life Sciences Mammalian female genital system MAPAS GENETICOS ovarian follicle Ovarian Follicle - chemistry OVARIAN FOLLICLES PCR Physiological aspects pig PORCIN Reverse Transcriptase Polymerase Chain Reaction - veterinary Sequence Homology, Nucleic Acid SWINE Swine - genetics Terrestrial animal productions Vertebrata Vertebrates Vertebrates: reproduction
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	cDNA clones from a pig granulosa cell cDNA library were isolated by differential hybridisation for follicle stimulating hormone (FSH) regulation in granulosa cells in a previous study. The clones that did not match any known sequence were studied for their expression in granulosa cells (treated or not by FSH) and in fresh isolated ovarian follicles mainly by comparative RT-PCR analysis. These results give functional data on genes that may be implicated in follicular growing. These ESTs [expressed sequence tags] have been localised on the porcine genome, using a somatic cell hybrid panel, providing new type I markers on the porcine map and information on the comparative map between humans and pigs.
ISSN:	0999-193X 1297-9686 1297-9686
DOI:	10.1186/1297-9686-33-1-69