Evaluating the conservation priority of key biodiversity areas based on ecosystem conditions and anthropogenic threats in rapidly urbanizing areas

•An assessment of conservation priorities for KBAs in highly urbanized regions was conducted.•Ecosystem conditions remain stable in most of KBAs over the past two decades.•Ecosystem conditions showed spatially significant negative correlations with anthropogenic threats.•Shenzhen Wutongshan is the highest priority KBA that needs to strengthen ecosystem conservation. The rapid and intense development in highly urbanized regions presents huge challenges to the conservation of biodiversity and ecosystem services. Progress on incorporating ecosystem conditions and anthropogenic threats into conservation assessments for key biodiversity areas (KBAs) in highly urbanized regions is still insufficient. To address this, this paper conducted an assessment of conservation priorities for key biodiversity areas in highly urbanized regions by integrating anthropogenic threats with ecosystem conditions. Taking 8 KBAs in the Guangdong - Hong Kong - Macao Greater Bay Area of China (GBA) as an example, this study found that the ecosystem condition of most of KBAs remains stable from 2000 to 2020. We found that the ecosystem conditions in most KBAs have not been negatively affected by anthropogenic threats. It is worth noting that Shenzhen Wutongshan (SZW) is the only KBA that faces a significant increase in anthropogenic threats with a significant decline in ecosystem condition during the past two decades. Overall, SZW is the highest priority KBA that needs to reduce the negative effects of anthropogenic threats and strengthen ecosystem conservation in the future.