Candiduria—Study of Virulence Factors and Its Antifungal Susceptibility Pattern in Tertiary Care Hospital
Abstract Background The increased incidence of candiduria in hospitalized patients is due to the use of indwelling devices, long-term antibiotics, parenteral nutrition, and immunocompromised status of the patient. In this study, an attempt was made to speciate, characterize, and determine the antifu...
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Veröffentlicht in: | Journal of laboratory physicians 2021-09, Vol.13 (3), p.231-237 |
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Sprache: | eng |
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Zusammenfassung: | Abstract
Background
The increased incidence of candiduria in hospitalized patients is due to the use of indwelling devices, long-term antibiotics, parenteral nutrition, and immunocompromised status of the patient. In this study, an attempt was made to speciate, characterize, and determine the antifungal susceptibility pattern of
Candida
isolated from urinary tract infections (UTIs).
Materials and Methods
A total of 70
Candida
isolates were obtained from urine samples. The isolated
Candida
species were studied for the production of virulence factors like phospholipase, protease activities, hemolysin, and biofilm production. Antifungal susceptibility testing of the isolated yeasts was done using Mueller-Hinton agar supplemented with 0.5 mg/mL methylene blue by E-test method for amphotericin B, fluconazole, caspofungin, and voriconazole.
Results
Out of 70 isolates,
Candida tropicalis
was the most frequently isolated species (65.7%), followed by
Candida albicans
(14.3%),
Candida glabrata
(7.1%),
Candida krusei
(5.7%),
Candida parapsilosis
(4.3%), and
Candida dubliniensis
(2.9%). A total of 37.1% were biofilm producers, 62.9% showed proteinase activity, 38.6% were phospholipase positive, and 58.6% isolates showed hemolytic activity. Antifungal susceptibility profile of
Candida
species showed 38.6, 25.7, 15.7, and 12.9% resistance to amphotericin B, fluconazole, caspofungin, and voriconazole, respectively.
Conclusion
A rising trend in isolation of non-albicans
Candida
from urinary isolates was noticed, which was statistically significant when comparing catheterized and noncatheterized urinary isolates from our study. However, there was no statistically significant difference when different virulence factor expressions were compared among
Candida
spp. isolated from catheterized and noncatheterized urinary samples. Due to this rise in non-albicans
Candida
species causing UTI that are intrinsically resistant to certain antifungal agents like azoles and increasing incidence of antifungal resistance, it is essential to monitor the antifungal susceptibility profile of
Candida
species causing candiduria. |
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ISSN: | 0974-2727 0974-7826 |
DOI: | 10.1055/s-0041-1730880 |