Comparison of N-Acetyl Cysteine and Resveratrol Treatments in Acetaminophen Toxicity Model in Rabbits

Purpose: Acetaminophen is safe analgesic drug but it may cause hepatic failure in high doses. N-acetyl cysteine (NAC) is used in the treatment of acetaminophen intoxication. Resveratrol may be used in experimental acetaminophen intoxication models. Purpose, compare the efficacy of NAC and resveratrol treatments in acetaminophen intoxication. Materials and Methods: We used 34 rabbits. Six rabbits were included in control group, and 7 rabbits were enrolled in other groups Asetaminophen (APAP) (N=7) group. The animals were administered 2 g/kg of acetaminophen by an orogastric tube. Asetaminophen+N-acetylcysteine (APAP+NAC) (N=7) group, 150 mg/kg NAC was administered intramuscular (i.m) to animals at 1st hour following 2 g/kg of acetaminophen administration by orogastric tube. Asetaminophen+resveratrol (APAP+RES) (N=7) group, animals received 100 mg/kg resveratrol by orogastric tube at 1st hour following oral administration of 2 g/kg of acetaminophen by orogastric tube. e. Asetaminophen+N-acetyl cysteine+resveratrol (APAP+NAC + RES) (N=7) group, animals received 100 mg/kg resveratrol by orogastric tube and 150 mg/kg NAC i.m at 1st hour following oral administration of 2 g/kg of acetaminophen by orogastric tube. ALT, AST, GSH, and acetaminophen levels were measured. Animals were sacrificed at the 24th hour. Liver samples were obtained for histopathological examination. Results: According to our results, the GSH level at 12th hour was significantly different between APAP+NAC group and APAP+RES group (p=0.007). In histopathological examination, HSCORE level we used for apoptosis was statistically significantly lower in control group than in APAP group (p=0.007). Its supports that acetaminophen toxicity triggers apoptosis in liver cells. Conclusion: Resveratrol may be an alternative treatment option to NAC in the treatment of acetaminophen toxicity.