The effect of Lactobacillus with prebiotics on KPC-2-producing Klebsiella pneumoniae

This study investigated the inhibitory effect of spp. with prebiotics against carbapenemase-2 (KPC-2)-producing using both experiments and animal models. Thirty-three spp. strains were confirmed by 16S rDNA sequencing, and four different PFGE genotyped KPC-2-producing strains were selected for investigation. studies, including broth microdilution assays, changes in pH values in lactobacilli cultures with different prebiotics, time-kill tests of spp. against KPC-2-producing and further alone or in combination with prebiotics against KPC-2-producing in an animal model, were performed. The lower pH value of the cell-free supernatant was associated with a lower minimal inhibitory percentage of the strain against KPC-2-producing Furthermore, lactulose/isomalto-oligosaccharide/inulin and fructo-oligosaccharide can enhance the inhibitory effect of all 10 CFU/ml strains against KPC001. Three strains (LYC1154, LYC1322, and LYC1511) that could be persistently detected in the stool were tested for their ability to reduce the amount of KPC001 in the feces individually or in combination. A significantly better effect in reducing the amount of KPC001 was observed for the combination of three different species than for each of them alone. Furthermore, their inhibitory effect was enhanced after adding lactulose or isomalto-oligosaccharide (both