Peribacillus castrilensis sp. nov.: A Plant-Growth-Promoting and Biocontrol Species Isolated From a River Otter in Castril, Granada, Southern Spain

A strictly aerobic, chemoheterotrophic, endospore-forming, Gram-positive, rod-shaped bacterial strain N3 T was isolated from the feces of a river otter in Castril (Granada, southern Spain). It is halotolerant, motile, and catalase-, oxidase-, ACC deaminase-, and C4- and C8-lipase-positive. It promotes tomato plant growth and can reduce virulence in Erwinia amylovora CECT 222 T and Dickeya solani LMG 25993 T through interference in their quorum-sensing systems, although other antagonistic mechanisms could also occur. A phylogenetic analysis of the 16S rRNA gene sequence as well as the phenotypic and phylogenomic analyses indicated that the strain N3 T is a novel species of the genus Peribacillus , with the highest 16S rRNA sequence similar to that of Bacillus frigoritolerans DSM 8801 T (99.93%) and Peribacillus simplex DSM 1321 T (99.80%). Genomic digital DNA–DNA hybridization (dDDH) between the strain N3 T and Bacillus frigoritolerans DSM 8801 T and Peribacillus simplex was 12.8 and 69.1%, respectively, and the average nucleotide identity (ANIb) of strain N3 T and Bacillus frigoritolerans DSM 8801 T and Peribacillus simplex was 67.84 and 93.21%, respectively. The genomic G + C content was 40.3 mol%. Its main cellular fatty acids were anteiso-C 15:0 and iso-C 15:0 . Using 16S rRNA phylogenetic and in silico phylogenomic analyses, together with the chemotaxonomic and phenotypic data, we demonstrated that the type strain N3 T (=CECT 30509 T = LMG 32505 T ) is a novel species of the genus Peribacillus and the name Peribacillus castrilensis sp. nov. is proposed.