Argon plasma improves the tissue integration and angiogenesis of subcutaneous implants by modifying surface chemistry and topography

Tissue integration and vessel formation are important criteria for the successful implantation of synthetic biomaterials for subcutaneous implantation. We report the optimization of plasma surface modification (PSM) using argon (Ar), oxygen (O ) and nitrogen (N ) gases of a polyurethane polymer to enhance tissue integration and angiogenesis. The scaffold's bulk and surface characteristics were compared before and after PSM with either Ar, O and N . The viability and adhesion of human dermal fibroblasts (HDFs) on the modified scaffolds were compared. The formation of extracellular matrix by the HDFs on the modified scaffolds was evaluated. Scaffolds were subcutaneously implanted in a mouse model for 3 months to analyze tissue integration, angiogenesis and capsule formation. Surface analysis demonstrated that interfacial modification (chemistry, topography and wettability) achieved by PSM is unique and varies according to the gas used. O plasma led to extensive changes in interfacial properties, whereas Ar treatment caused moderate changes. N plasma caused the least effect on surface chemistry of the polymer. PSM-treated scaffolds significantly (