Z-chromosome specific primers for chicken-quail hybrid blastoderm

Z-chromosome specific primers for chicken-quail hybrid blastoderm

A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail b...

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Veröffentlicht in:The Journal of Poultry Science 2007, Vol.44(2), pp.209-212
Hauptverfasser: Takagi, S.(Nagoya Univ. (Japan)), Ono, T, Tsukada, A, Atsumi, Y, Mizushima, S, Saito, N, Shimada, K
Format: Artikel
Sprache:eng
Schlagworte:
ADN
CAILLE
chicken
CHICKENS
CHROMOSOME
CHROMOSOMES
CODORNIZ
CROMOSOMAS
DNA
GENE
GENES
GENOMAS
GENOME
GENOMES
PCR
PKCI
POLLO
POULET
quail
QUAILS
Z-chromosome
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Beschreibung
Zusammenfassung:A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail but not chickens. These chicken and quail primers validly identified chicken-quail hybrids derived from Z-chromosome carrying sperm of chicken and the hybrid derived from Z-chromosome carrying oocyte of quail, respectively.
ISSN:1346-7395
1349-0486
DOI:10.2141/jpsa.44.209