Recent advances in PCR-free nucleic acid detection for SARS-COV-2

As the outbreak of Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory disease coronavirus 2 (SARS-COV-2), fast, accurate, and economic detection of viral infection has become crucial for stopping the spread. Polymerase chain reaction (PCR) of viral nucleic acids has been the gold...

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Veröffentlicht in:Frontiers in bioengineering and biotechnology 2022-10, Vol.10, p.999358
Hauptverfasser: Ma, Xiaowei, Xu, Jingsong, Zhou, Fei, Ye, Jing, Yang, Donglei, Wang, Hua, Wang, Pengfei, Li, Min
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Sprache:eng
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Zusammenfassung:As the outbreak of Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory disease coronavirus 2 (SARS-COV-2), fast, accurate, and economic detection of viral infection has become crucial for stopping the spread. Polymerase chain reaction (PCR) of viral nucleic acids has been the gold standard method for SARS-COV-2 detection, which, however, generally requires sophisticated facilities and laboratory space, and is time consuming. This review presents recent advances in PCR-free nucleic acid detection methods for SARS-CoV-2, including emerging methods of isothermal amplification, nucleic acid enzymes, electrochemistry and CRISPR.
ISSN:2296-4185
2296-4185
DOI:10.3389/fbioe.2022.999358