Commentary: Herpes keratitis: A diagnostic challenge

[8],[9] Virus isolation, though considered a 'gold standard' for diagnosis of viral infections, is time-consuming, has low sensitivity, and requires a special laboratory for viral processing. Multiplex PCR allows for simultaneous amplification of multiple target sequences in a single tube using specific primer sets in combination with probes labeled with spectrally distinct fluorophores. [13] The study emphasizes the role of multiplex RT-PCR for the detection of HSV and varicella zoster (VZV) DNA in corneal scrapings of suspected herpes keratitis in an ocular microbiology laboratory.