Microscopic and physicochemical evaluation of Ruta angustifolia leaves

There are thousands of species of known medicinal plants in the world. L. has been widely used in traditional medication for jaundice and liver disease. Previous studies have shown that leaves can inhibit the hepatitis C virus in Huhit culture cells, reduce the value of NS3 protein, and possess a synergistic effect in combination with antiviral drugs. Therefore, this plant is potential to be developed as a drug candidate. Characteristics of plants including microscopic, physicochemical properties, and phytochemical profiles are necessary information to ensure the quality of raw material in drug development. This study was carried out to examine the microscopic and physicochemical including the standardized parameter of leaves to fulfil the quality raw materials as traditional medicine. Simplicia of leaves obtained from Jombang, East Java, were observed a microscope and determined its physicochemical properties referred to the Materia Medica Indonesia V. The TLC and HPLC profiles of extract were determined as well. Microscopic analysis were conducted by transfection sections and the presence of epidermis cells, palisade, mesophyll with stomata, and Ca-oxalate crystal were found. The standard parameter obtained value of loss of drying, extractive value in water and ethanol, and ash value. The TLC and HPLC profiles of leaves extract demonstrated to contain with flavonoid, terpenoids, and alkaloids. obtained from Jombang, east Java, has a specific character in microscopic analysis. The physicochemical properties analysis of leaves as a raw material met the requirements according to Materia Medica Indonesia V.