Physiological Consequences of Genetic Transformation: Result of Target Gene Expression or Stress Reaction?

The transgenic and non-transgenic tobacco cell cultures were analyzed for resistance to abiotic and biotic stress. The different physiological reaction of cell culture depending on T-DNA structure (or transgen structure) was observed. The cell culture transformed by disarmed Agrobacterium tumefaciense A699 with pCNL 65 nptII demonstrated the same stress-resistance as non-transgenic control cell culture. The cell culture transformed by Agrobacterium tumefaciense LBA 4400 pBiCaMV nptII + hsp101 showed a raised stress-resistance to high temperature, high KF concentration, and to the action of Clavibacter michiganensis ssp sepidonicus. Obviously, the expression of transferred arabodopsis gene hsp101 provides protection properties of transgenic cell culture under the influence of various stress factors. Moreover, that agrobacterial transformation as previous stress-factor is supposed to make a contribution to formation of transgenic cell culture cross-resistance.