Purification and characterization of beta-1, 3-glucanase from the secretion of Simira glaziovii colleters (Rubiaceae)

In this study, beta-1,3-glucanase was isolated from Simira glaziovii secretion. The purification process was achieved by a combination of chromatographic methods and was analyzed by SDS-PAGE. The purified enzyme presented an estimated molecular mass of 35 kDa. The optimum pH of enzyme was 5.2 Uma beta-1,3-glucanase foi purificada a partir da secreção de Simira glaziovii, através de vários processos cromatográficos, tendo a análise do perfil protéico acompanhado de SDS-PAGE. A enzima purificada apresentou uma massa molecular estimada de 35 kDa. O pH ótimo obtido para a enzima foi de 5,2.