Biological study for virulence factor Haemolycin A in E. coli Bactria Causes urinary tract infection

This study was carried out to isolated and diagnoses E. Coli bacteria case infection of the urinary tract also study virulence factor Haemolysin A in the isolated more than resistance to antibiotic. This aim of the study 200 sample of urinc were collected from patients in Ramadi teaching hospital (male and female) of different age (one year – 70 years). the E. Coli bacteria were the most spreading type in the case of urinary tract infection since they were isolated with percentage 59% out the total number of bacterial isolates, indentification the disc diffusion method was used test the isolate towards (10) ten antibiotics this work also includes the study of some virulenece factor of isolated bacteria. The result revealted that a high ratio of bacteria which are gram negative E. Coli isolated from urinary tract infection showed high resistance to the antibiotic particularly cefotaxim 100%. Naldic acid 95.3% Gentimicin 97.2%. ten isolatedwere chosen from each bacteria types for the sake of studying their genetics homogeneity. DNA was extracted from ten isolated of each of the following bacteria E. coli this extracted DNA was used in multiplex (PCR) technique by using gen of Haemolysin. A. This result found one coloney this carries the gen (Hyl A_ number (7) to DNA genome sample. While the other result fond (Hyl A) for plasmed DNA in the samples (3 -6- 7).