Coupling of MBP fusion protein cleavage with sparse matrix crystallization screens to overcome problematic protein solubility

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Veröffentlicht in:	BioTechniques 2005-10, Vol.39 (4), p.476-480
Hauptverfasser:	Gruswitz, Franz, Frishman, Mary, Goldstein, Barry M, Wedekind, Joseph E
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology - instrumentation Biotechnology - methods Carrier Proteins - chemistry Crystallography, X-Ray Diverse techniques Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Humans Hydrogen-Ion Concentration IMP Dehydrogenase - chemistry Maltose-Binding Proteins Molecular and cellular biology Protein Structure, Tertiary Recombinant Fusion Proteins - chemistry Software Solubility
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