Three New Butenolides from the Fungus Aspergillus sp. CBS-P-2

Three new butenolides aspernolides H-J ( - ) together with seven known ones ( - ) were isolated from the fungus sp. CBS-P-2. Their chemical structures were established on the basis of 1D- and 2D-NMR spectroscopic data, HR-ESI-MS analysis, and their absolute configuration were determined by circular dichroism (CD) analysis. All the compounds were evaluated for the antioxidant effects by DPPH and ABTS methods, the antitumor activities against four human tumor cell lines (HL-60, ASPC1, HCT-116 and PC-3) and antimicrobial activities. Compounds - showed significant activity against DPPH (IC = 15.9-34.3 μM) and compounds - exhibited significant ABTS free radical scavenging activity (IC = 2.8-33.1 μM). Compounds , and showed potent cytotoxic activities against HL-60 cell lines with IC values of 39.4, 13.2 and 16.3 μM, respectively. Compound showed good antimicrobial activity against with minimum inhibitory concentration (MIC) of 21.3 μM.