RNA-seq analysis on cisplatin down-regulating expression of DRG neurons' axon guidance related genes

Objective To explore the effect of cisplatin on the expression of axon guidance related gene in mouse dorsal root ganglion (DRG) neurons using transcriptome sequencing technique, and to further explore the neurotoxic of cisplatin. Methods The primary cultures of DRG neurons was isolated from Kunming mice and divided into control group and injury group. Neurons in the control group were cultured for 24 hours, and those in the injury group were pre-cultured for 4 hours and then treated with 20 μmol/L cisplatin for 20 hours. To observe the neurites and the growth cones in two groups by the NF200 immunofluorescence staining. Total RNA was extracted from the cells, and transcriptome sequencing was performed using RNA-seq technology. The axon guidance related genes were searched through literature and database (total 231), and the differential expression of related genes was analyzed. Real-time fluorescence quantitative PCR (qPCR) was used to verify the expression of related genes (Cxcl12,Ext1,Gdnf,Plxna4,Nrp2,Apbb